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Expression of NDRG2 during colon carcinoma cells differentiation induced by sodium butyrate and its significance
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 1
- DOI:
- 10.3321/j.issn:1000-2790.2009.01.005
- Key Word:
- NDRG2基因;丁酸钠;结肠肿瘤;细胞分化
Abstract： 目的:研究丁酸钠诱导结肠癌细胞分化过程中抑癌候选基因NDRG2的表达特点及意义.方法:采用丁酸钠诱导结肠癌HT29,SW480,SW620细胞分化,通过碱性磷酸酶(AKP)测定和透射电镜评价结肠癌细胞的分化程度,应用Real time BT-PCR和Western Blot法测定不同分化点(0,1,2,3,4,5,6 d)NDRG2 mRNA和蛋白的表达特点.结果:经丁酸钠作用24 h-6 d后,结肠癌细胞中NDRG2基因mRNA和蛋白水平均显著高于对照组水平.结论:丁酸钠诱导结肠癌细胞分化过程中NDRG2表达显著升高,提示NDRG2是参与结肠癌细胞分化的相关基因.
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