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Localization and clone of four segments of highly conserved sequence of βig-h3 gene and construction of their prokaryotic expression vector
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 1
- DOI:
- 10.3321/j.issn:1000-2790.2009.01.002
- Key Word:
- βig-h3;高度保守序列;原核表达载体
Abstract： 目的:确定βig-h3基因4段高度保守序列的序列位置,克隆βig-h3全长及4段高度保守序列的基因,分别构建原核表达载体.方法:用RT-PCR方法获得βig-h3全长及4段高度保守序列的基因,以pRSET-A为载体,分别构建重组原核表达质粒.结果:测序结果证实获得βig-h3全长及4段高度保守序列的基因,其序列与GenBank中报道完全一致.分别正确连入pRSET-A载体中,读码框正确.结论:成功构建βig-h3基因全长及4段高度保守序列的原核表达载体,为进行结构与功能相关性研究奠定了基础.
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