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Transplantation of olfactory ensheathing cells seeded in linearly oriented nerve guidance scaffolds promotes axon regeneration in spinal cord injury
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 21
- DOI:
- 10.3321/j.issn:1000-2790.2008.21.002
- Key Word:
- 脊髓损伤;嗅鞘细胞;硫酸肝素;胶原蛋白;神经组织工程;轴突再生
Abstract： 目的:观测嗅鞘细胞复合于定向性硫酸肝素-胶原蛋白支架材料,对移植修复脊髓损伤的疗效.方法:采用胶原蛋白和硫酸肝素在冷凝条件下制备具有纵行、平行排列微管的硫酸肝素-胶原蛋白基质材料.将嗅鞘细胞与硫酸肝素-胶原蛋白支架材料复合,构建新型神经组织工程材料,移植修复大鼠3 mm节段性脊髓损伤.移植术后12 wk经神经学观察、电生理学、形态学检测其修复疗效.结果:NF,GAP-43免疫荧光双标和甲苯氨兰染色、电镜观察证实脊髓再生神经纤维成功地长入该神经组织工程材料内.经BBB评分和电生理学检测,部分大鼠运动功能恢复良好.结论:以嗅鞘细胞复合于定向性硫酸肝素-胶原蛋白支架材料,并移植修复脊髓损伤,可以促进脊髓轴突的再生.
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