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Increase of mouse p38 MAPK phospho-rylation in brain regions related to ad-diction,learning ＆ memory by prena-tal heroin exposure
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 18
- DOI:
- 10.3321/j.issn:1000-2790.2008.18.004
- Key Word:
- 海洛因;p38丝裂原活化蛋白激酶;前额叶皮层;海马;伏核;杏仁核
Abstract： 目的:观察海洛因暴露对小鼠成瘾和学习记忆相关脑区的磷酸化p38 MAPK(p-p38)表达的影响,探讨p38 MAPK细胞信号转导通路是否参与了发育早期海洛因暴露引起子代脑发育损伤的分子机制.方法:于胚胎鼠龄9～18 d时给孕鼠皮下注射海洛因10 mg/(kg·d),建立子宫内海洛因暴露的小鼠模型.按出生前处理将小鼠分为海洛因和盐水组.蛋白免疫印迹检测两组小鼠前额叶皮层、海马、伏核、杏仁核脑区P-p38的表达改变.结果:与盐水组相比,海洛因组小鼠的p-p38蛋白表达在前额叶皮层、海马、伏核、杏仁核4个脑区均有明显增加(P＜0.05).结论:海洛因暴露可引起小鼠前额叶皮层、海马、伏核、杏仁核脑区的P-p38 MAPK蛋白表达上调,提示p38 MAPK信号通路可能参与了海洛因损伤小鼠神经发育的分子机制.
- [1]Peifer C,Wagner G,Laufer S.New Approaches to the Treatment of Inflammatory Disorders Small Molecule Inhibitors of p38 MAP Kinase.[J].2006,6(2).
- [2]J, Yanai,R A, Steingart,N, Snapir,etc.The relationship between neural alterations and behavioral deficits after prenatal exposure to heroin.[J].2000,914.
- [3]Moe V,Dale AM,Due-Tonnessen P,etc.Volumetric cerebral characteristics of children exposed to opiates and other substances in utero.[J].2007,36(4).
- [4]JOSEPH YANAI,TAMAR L. BEN-SHAANAN,HANA HAIMOVITCH,etc.Mechanism-Based Approaches for the Reversal of Drug Neurobehavioral Teratogenicity[J].2006,1074(aug).
- [5]Yanai J,Huleihel R.Disruption of the development of cholinergic-induced translocation/activation of PKC isoforms after prenatal heroin exposure.[J].2006,69(2).
- [6]Sanjay, Kumar,Jeffrey, Boehm,John C, Lee.p38 MAP kinases: key signalling molecules as therapeutic targets for inflammatory diseases.[J].2003,2(9).