You Position: Home > Paper
Activation of GABABR1 neurons in hypothalamic paraventricular nucleus of rat by lipopolysaccharide
( views:56, downloads:4 )
- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 16
- DOI:
- 10.3321/j.issn:1000-2790.2008.16.002
- Key Word:
- 脂多糖类;基因,Fos;γ-氨基丁酸B受体亚单位1(GABABR1);荧光抗体技术;下丘脑室旁核(PVN);应激
Abstract： 目的:探讨大鼠下丘脑室旁核(PVN)-氨基丁酸B受体亚单位1(GABABR1)阳性神经元对脂多糖(LPS)刺激的反应. 方法:将大鼠腹腔注射LPS建立免疫应激模型,对照组注射等量的生理盐水,采用免疫荧光双标记与激光共聚焦显微镜技术,观察大鼠下丘脑室旁核内神经元Fos和GABABR1的标记情况以及它们在同一神经元内是否有共标记. 结果:腹腔注射LPS可使大鼠PVN内表达Fos神经元数量显著增高,且PVN内部分神经元可同时被Fos和GABABR1双重标记,双重标记的神经元大约占Fos神经元的30%,占GABABR1的24%. 结论:下丘脑室旁核部分GABABR1阳性神经元参与了LPS诱导的免疫应激反应,它们可能在下丘脑-垂体-肾上腺轴的调节方面起重要作用.
- [1]杨志军,葛煦,饶志仁,等.Fos蛋白在LPS免疫激发大鼠脑内的分布[J].2000,(4).
- [2]袁莲芳,赵钢,贾宏阁.大鼠下丘脑室旁核GABAARα1阳性神经元参与内毒素诱导的免疫应激反应[J].2008,(3).
- [3]路翠艳,潘芳.应激反应中HPA轴的中枢调控和免疫调节[J].2003,(3).
- [4]Cullinan WE.GABA(A) receptor subunit expression within hypophysiotropic CRH neurons: a dual hybridization histochemical study.[J].2000,419(3).
- [5]Chantal Decavel,Anthony N. Van Den Pol.GABA: A dominant neurotransmitter in the hypothalamus[J].1990,302(4).
- [6]Emson PC,Billinton A,Marshall FH,etc.Advances in the molecular understanding of GABA(B) receptors.[J].2001,24(5).
- [7]R L, Macdonald,R W, Olsen.GABAA receptor channels.[J].1994,17.
- [8]O'Connor KA,Maier SF,Johnson JD,etc.The role of IL-1beta in stress-induced sensitization of proinflammatory cytokine and corticosterone responses.[J].2004,127(3).
- [9]Hadid R,Giacomini M,Chautard T,etc.Role of several mediators of inflammation on the mouse hypothalamo-pituitary-adrenal axis response during acute endotoxemia.[J].1999,6(5).
- [10]De-Pei Li,Hui-Lin Pan.Role of gamma-Aminobutyric Acid (GABA)_A and GABA_B Receptors in Paraventricular Nucleus in Control of Sympathetic Vasomotor Tone in Hypertension[J].2007,320(2).
- [11]Maier SF.Bi-directional immune-brain communication: Implications for understanding stress, pain, and cognition.[J].2003,17(2).
- [12]Villalba RM,Alvarez FJ,Stern JE,etc.Expression of GABA Receptors in Magnocellular Neurosecretory Cells of Male, Virgin Female and Lactating Rats.[J].2005,17(7).