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Effect of electromagnetic fields on transcript levels of apoptosis-related genes in embryonic stem cell-derived neural progenitor cells
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 10
- DOI:
- 10.3321/j.issn:1000-2790.2008.10.004
- Key Word:
- 小鼠;胚胎;干细胞;低频率电磁场;基因,bel-2;bax;GADD45
Abstract： 目的:利用小鼠胚胎干(ES)细胞分化成神经前体细胞模型,研究极低频率电磁场(ELF-EMF)对细胞周期调节基因、凋亡相关基因以及细胞增生和凋亡的影响.方法:分离、培养和诱导ES成神经前体细胞,于诱导后第4日起,实验组予ELF-EMF干预,对照组采用假暴露,于4+4 d,4+7 d,4+11 d,4+17 d,4+23 d使用BrdU和Nestin免疫荧光染色检测细胞生长分化,RT-PCR检测bel-2,bax,GADD45基因转录产物水平.结果:对照组和实验组的BrdU和Nestin免疫荧光染色未能发现细胞生长和分化有明显改变,对照组和实验组的RT-PCR检测发现,在4+11 d时bcl-2和bax mRNA表达升高(P<0.01;P<0.05).GADD45转录水平在终末期(4+23 d)下降(P<0.01).结论:EMF能够暂时性地影响ES源性神经前体细胞凋亡和细胞周期调控相关基因的转录水平,但这些改变不足以使细胞发生可检测到的细胞生理上改变.
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