You Position: Home > Paper
Promoting role of liver X receptor activation in synthesis of neurosteriod in primary hippocampal neurons
( views:138, downloads:16 )
- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 10
- DOI:
- 10.3321/j.issn:1000-2790.2008.10.002
- Key Word:
- 肝X受体;P450侧链裂解酶;神经甾体;海马,神经元
Abstract： 目的:研究肝X受体(LXR)激活对海马神经元神经甾体合成的影响.方法:将大鼠海马神经元体外培养至第7日,在培养液中加入2.0μmol/L T0901317,继续培养48 h.应用高效液相.质谱联用(HPLC-MS)分离测定培养液中游离型神经甾体孕烯醇酮(PREG)、脱氢表雄酮(DHEA)和结合型神经甾体孕烯醇酮硫酸酯(PREGS)、脱氢表雄酮硫酸酯(DHEAS)的含量;RT-PCR方法观察海马神经元P450侧链裂解酶(P450scc)、甾体合成快速调节蛋白(StAR)和3β-羟基甾醇脱氢酶(3β-HSD)等神经甾体合成关键酶基因的mRNA的表达.结果:T0901317激活LXR,促进P450scc,StAR和3β-HSD的mRNA表达,增加海马神经元培养液中神经甾体PREG,DHEA,PREGS和DHEAS含量.结论:LXR激活时,可通过上调海马神经元P450scc,StAR和3β-HSD的mRNA表达.促进神经甾体的合成.
- [1]张翠欣,侯艳宁.乙醇、乙醛对原代培养星形胶质细胞神经甾体合成的影响[J].2006,(5).
- [2]Hige T,Fujiyoshi Y,Takahashi T.Neurosteroid pregnenolone sulfate enhances glutamatergic synaptic transmission by facilitating presynaptic calcium currents at the calyx of Held of immature rats.[J].2006,24(7).
- [3]Staufenbiel M,Wolfe D,Lazo JS,etc.The liver X receptor ligand T0901317 decreases amyloid beta production in vitro and in a mouse model of Alzheimer's disease[J].2005,280(6).
- [4]Pianos A,Sazdovitch V,Delacourte A,etc.Neurosteroid Quantification in Human Brain Regions: Comparison between Alzheimer's and Nondemented Patients.[J].2002,87(11).
- [5]Furuya K,Dai XN,Chen L,etc.Chronic DHEAS administration facilitates hippocampal long-term potentiation via an amplification of Src-dependent NMDA receptor signaling.[J].2006,51(3).
- [6]主编司徒镇强, 吴军正.细胞培养[M].2007.