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Relationship between receptor expression on cell surface of several solid tumors and tumor sensitivity to IFN-α 2a
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 10
- DOI:
- 10.3321/j.issn:1000-2790.2008.10.001
- Key Word:
- 干扰素α;受体,干扰素α;敏感性与特异性
Abstract： 目的:探讨肿瘤细胞表面干扰素α受体的表达与其肿瘤对IFN-α 2a治疗敏感性之间的关系.为建立干扰素α敏感肿瘤的快速评价方法奠定基础.方法:MTT法体外筛选IFN-α 2a敏感与抗性肿瘤细胞株,选取人肺腺癌细胞A549,SPC-A-1,GLC-82和人低分化胃腺癌细胞MKN-45共4株细胞;通过构建荷瘤裸鼠模型,证实不同肿瘤对干扰素治疗的敏感性存在差异.流式细胞术、免疫组织化学、Western Blot检测肿瘤细胞表面IFN-α受体的表达差异.结果:体内、体外实验结果表明:A549,SPC-A-1为IFN-α2a敏感性细胞株,MKN-45,GLC-82为IFN-α 2a抗性细胞株.细胞和组织水平检测的结果表明:表达量由高到低依次为A549,SPC-A-1,MKN-45,GLC-82.结论:IFN-α受体表达的高低与肿瘤细胞对干扰素的敏感性具有密切的相关性,对实现有针对性的个体化治疗具有一定的指导意义.
- [1]Neubauer A,Burchert A.Interferon alpha and T-cell responses in chronic myeloid leukemia.[J].2005,46(2).
- [2]Jonasch E,Haluska FG.Interferon in oncological practice: review of interferon biology, clinical applications, and toxicities.[J].2001,6(1).
- [3]Badgwell B,Becknell B,Magro C,etc.The antitumor effects of IFN-alpha are abrogated in a STAT1-deficient mouse.[J].2003,112(2).
- [4]Carson W 3rd,Skaf A,Lesinski GB,etc.The antitumor effects of interferon-alpha are maintained in mice challenged with a STAT1-deficient murine melanoma cell line.[J].2004,116(1).
- [5]JE Darnell,Jr,IM Kerr,etc.Jak-STAT pathways and transcriptional activation in response to IFNs and other extracellular signaling proteins[J].1994,264 (5164).