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Cloning and DNA sequence analysis of anti-cTnI murine antibody Fab fragment
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 9
- DOI:
- 10.3321/j.issn:1000-2790.2008.09.005
- Key Word:
- 抗体;单克隆;基因;序列分析;血清心肌钙蛋白
Abstract： 目的:克隆鼠抗人cTnI mAb Fab段基因并进行序列分析.方法:设计扩增鼠IgG重链Fd段及K轻链引物,从分泌cTnI mAb的杂交瘤细胞中提取总RNA,RT-PCR扩增,对扩增产物进行分子克隆、测序及序列分析.结果:重链和轻链引物分别扩增出一约700 bp和800 bp DNA片段.经序列分析,与已发表的鼠IsG基因序列对比,其核苷酸及其所推导的氨基酸序列符合鼠IgG1 Fab段特征.在GenBank登录,登录号为AY484430(重链),AY484431(轻链);氨基酸序列登录号为AAR83243(重链),AAR83244(轻链).结论:本实验室获得了完整的鼠源性抗人cTnI mAb Fab段基因,为鼠抗人cTnI的人源化改造奠定了基础.
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