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Intervention of ligustrazine on expressions of VIII collagen and p53 in vascular smooth muscle cells of canine LAD after CABG in dogs
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 5
- DOI:
- 10.3321/j.issn:1000-2790.2008.05.004
- Key Word:
- 冠状动脉狭窄;血管平滑肌细胞;川芎嗪;VIII型胶原;p53;基因表达
Abstract： 目的:探讨川芎嗪(LG)对冠状动脉旁路移植术(CABG)后冠脉左前降支(LAD)血管平滑肌细胞表达VIII型胶原和p53反应性改变的影响. 方法:45只健康杂交犬体质量(17.9±2.2) kg,结扎LAD近段制作局部冠状动脉狭窄模型后,将存活的28只犬分为LG组(n=10),维拉帕米(VR)组(n=10)和生理盐水(CN)对照组(n=8),三组均行CABG,手术前后对各组犬施加不同的药物干预. 经犬右心耳抽血检测不同时段血浆NO含量变化;术后2 wk切取局部LAD, 用Western Blot法检测LAD组织匀浆VIII型胶原和p53蛋白表达,并用免疫组化方法观察两种分子在局部的原位表达情况. 结果:吻合血管后血浆NO含量60 min时,LG组下降至(77.7±2.4) μmol/L,VR组为(69.3±1.5) μmol/L,CN对照组为(62.4±2.1) μmol/L,LG组与其他两组相比较差异具有统计学意义(P<0.05);三组正常LAD的组织匀浆表达VIII 型胶原和p53水平低,VR组和CN对照组的血管吻合处远段LAD表达VIII 型胶原增加比川芎嗪组明显,而LG组p53表达增加比其他两组明显. 免疫组化方法检测表明:三组的吻合处远段血管片段局部VIII 型胶原表达明显增加,LG组明显弱于其他两组,LG组可以检测到p53表达,而其他两组吻合后损伤血管的p53无明显表达. 结论:LG可抑制CABG后血管内皮损伤及LAD平滑肌细胞表达VIII胶原反应性的上调和促进p53表达,表明其可能具有防治CABG术后再狭窄的作用.
- [1]兰海,王臻,张琳西,等.川芎嗪抑制瘢痕成纤维细胞增殖及胶原的合成[J].1999,(11).
- [2]何维来,陈如坤,周汝元.川芎嗪对结扎犬LAD损伤冠脉内皮及心肌的保护作用[J].2005,(23).
- [3]John, Mercer,Nichola, Figg,Victoria, Stoneman,etc.Endogenous p53 protects vascular smooth muscle cells from apoptosis and reduces atherosclerosis in ApoE knockout mice.[J].2005,96(6).
- [4]S M, Sanz-González,E, Poch,I, Pérez-Roger,etc.Control of vascular smooth muscle cell growth by cyclin-dependent kinase inhibitory proteins and its implication in cardiovascular disease.[J].2000,5.
- [5]J, Linde,B H, Strauss.Pharmacological treatment for prevention of restenosis.[J].2001,6(2).
- [6]Rohit, Khurana,Zhenwu, Zhuang,Shalini, Bhardwaj,etc.Angiogenesis-dependent and independent phases of intimal hyperplasia.[J].2004,110(16).
- [7]Jian H,Yu D,Shengqi W,etc.Preparation of cardiovascular disease-related genes microarray and its application in exploring ligustrazine-induced changes in endothelial gene expression.[J].2004,56(4).
- [8]Bramucci E,Marziliano N,Rossi ML,etc.Phenotype commitment in vascular smooth muscle cells derived from coronary atherosclerotic plaques: differential gene expression of endothelial nitric oxide synthase[J].2005,49(1).
- [9]J, Sato,K, Nair,J, Hiddinga,etc.eNOS gene transfer to vascular smooth muscle cells inhibits cell proliferation via upregulation of p27 and p21 and not apoptosis.[J].2000,47(4).
- [10]Thomas N, Wight,Mervyn J, Merrilees.Proteoglycans in atherosclerosis and restenosis: key roles for versican.[J].2004,94(9).