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Protective effect of HSP90 against heat injury on human dermal fibroblasts in vitro
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 4
- DOI:
- 10.3321/j.issn:1000-2790.2008.04.002
- Key Word:
- 成纤维细胞;热损伤;热休克蛋白质90;保护
Abstract： 目的:探讨人真皮成纤维细胞热损伤变性后HSP90的保护作用.方法:培养的人真皮成纤维细胞在52℃,30 s热水损伤24 h后用免疫荧光观察HSP90表达部位的改变并与正常组比较,用Western Blot 进一步分析正常的FB和热变性的FB在3, 6, 12, 24, 48, 72, 96及120 h时相点HSP90表达量的改变.结果:用免疫荧光观察到FB热损伤后24 h胞质胞核中出现HSP90高表达.Western Blot显示3 h即有HSP90表达量升高,24 h达到高峰,维持到96 h,120 h恢复到正常.结果:FB热变性可以引起HSP90异常高表达,HSP90对FB热损伤具有保护作用.
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