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Feasibility of magnetic nanoparticles modified by PEI as gene vector
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 1
- DOI:
- 10.3321/j.issn:1000-2790.2008.01.005
- Key Word:
- 磁性;纳米粒子;转染;磁场
Abstract： 目的:评价氧化铁磁性纳米颗粒(MNPs)作为体外基因载体的可行性及外加磁场对于其转染效率的影响.方法:以FeCl3水溶液作原料,采用部分还原沉淀法制备纳米磁性颗粒,并用聚乙烯亚胺(PEI)对其进行表面修饰.利用电镜、X-射线衍射、琼脂糖凝胶电泳等手段对MNPs的形貌、粒度分布、物相组成、DNA结合能力等进行表征.应用荧光素酶报告基因系统,检测MNPs-PEI将外源基因转染至不同细胞系的转染特性以及外加磁场对基因载体效率的影响.结果:MNPs-PEI可将外源基因转染至多个细胞系并高效表达.不同细胞系的转染效率和时间各不相同.外加磁场可使转染效率提高4～5倍.结论:PEI修饰的磁性纳米颗粒是一种可用于体外转染的新型非病毒基因载体,外加磁场可提高其转染效率.
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