Preventive and therapeutic effects of hyperbaric oxygen and hypobaric hypoxia preconditioning on cerebral infarction after permanent middle cerebral artery occlusion in rabbits and its mechanism
- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 23
- DOI:
- 10.3321/j.issn:1000-2790.2007.23.001
- Key Word:
- 高压氧;缺氧;局灶性脑缺血
Abstract： 目的:观察高压氧预处理(HBOP)和低压缺氧预处理(HHP)对兔大脑中动脉闭塞(MCAO)后局灶性脑梗死程度及周围脑组织氧分压(PbtO2)的影响.方法:雄性家兔72只随机分为单纯MCAO组,HBOP+MCAO组(100%O2,2.5ATA,1 h/d,5 d)和HHP+MCAO组(4000 m,61.6 kPa,3d),每组24只,采用改良Bederson法建立家兔MCAO模型,分别于MCAO前、MCAO后1,3和10 d观察脑MRI信号强度、脑梗塞灶体积及梗塞灶边缘PbtO2.结果:MRI结果显示MCAO后3,10 d时HBOP+MCAO组和HHP+MCAO组缺血区异常信号范围较单纯MCAO组明显缩小,HBOP+MCAO组缺血区标准化信号强度(SIR)于3,10 d时分别为1.62±0.09,1.17±0.10;HHP+MCAO组缺血区SIR于3,10 d时分别为1.64±0.06,1.20±0.09;均低于单纯MCAO组的1.83±0.07,1.36±0.06(P＜0.05).TTC染色显示的梗塞灶体积在3和10 d时HBOP+MCAO组和HHP+MCAO组小于单纯MCAO组;HBOP+MCAO组家兔梗塞灶边缘PbtO2于1,3和10 d时分别为(8.27±0.58),(11.92±1.27)和(16.12±1.42)mmHg;HHP+MCAO组家兔梗塞灶边缘PbtO2分别为(8.46±0.90),(10.77±1.19)和(15.33±1.29)mmHg,均高于单纯MCAO组的(7.03±1.06),(8.43±0.86)和(11.83±1.28)mmHg(P＜0.05).结论:HBOP及HHP对家兔MCAO后局灶性脑缺血具有一定的防治作用.
- [1]胡胜利,金清东,刘智,等.高压氧预适应对大鼠高原颅脑损伤的影响[J].2006,(6).
- [2]胥全宏,冯华,王宪荣,等.高原大鼠颅脑损伤局部脑组织氧分压变化特点[J].2005,(2).
- [3]Kojiro Wad,Masataka Ito,Takahito Miyazawa,etc.Repeated hyperbaric oxygen induces ischemic tolerance in gerbil hippocampus[J].1996,740(1-2).
- [4]Jantze H,Krug M,Zerbe R,etc.The importance of free hydroxyl radicals to hypoxia preconditioning.[J].2000,868(1).
- [5]Bederson J. B.,Pitts L. H.,Tsuji M.,etc.Rat middle cerebral artery occlusion: evaluation of the model and development of a neurologic examination[J].1986.
- [6]Duan C.-L.,Yan F.-S.,Song X.-Y.,etc.Changes of Superoxide Dismutase, Glutathione Perioxidase and Lipid Peroxides in the Brain of Mice Preconditioned by Hypoxia[J].1999,8(4-5).
- [7]Liu J,Yu F,Chan PH,etc.Neuroprotection by hypoxic preconditioning involves oxidative stress-mediated expression of hypoxia-inducible factor and erythropoietin.[J].2005,36(6).
- [8]Hakim A. M..Ischemic penumbra: The therapeutic window[J].1998.
- [9]Kim CH,Kim GT,Park JW,etc.Hyperbaric oxygenation pretreatment induces catalase and reduces infarct size in ischemic rat myocardium.[J].2001,442(4).
- [10]Schneider D,Henninger N,Gunther A,etc.Neuroprotective effect of hyperbaric oxygen therapy monitored by MR-imaging after embolic stroke in rats.[J].2006,201(2).
- [11]Astrup J.,Symon L.,Branston N. M.,etc.Cortical evoked potential and extracellular K+ and H+ at critical levels of brain ischemia[J].1977.