You Position: Home > Paper
Identification and analysis of a novel human NFBD1 promoter
( views:152, downloads:8 )
- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 22
- DOI:
- 10.3321/j.issn:1000-2790.2007.22.002
- Key Word:
- NFBD1;启动区(遗传学);增强子元件(遗传学);转录调控
Abstract： 目的:鉴定分析NFBD1启动子,为进一步研究其转录调控奠定基础.方法:综合应用生物信息学分析和异构体特异性的巢式RT-PCR技术鉴定NFBD1转录起始位点和转录变异体;高保真PCR方法扩增NFBD1基因启动子区域并分别定向克隆入pGL3-basic和pGL3-enhancer载体,构建NFBD1启动子荧光素酶报告基因重组体;荧光素酶分析检测启动子活性;生物信息学方法预测转录因子结合位点.结果:鉴定了一个新的NFBD1转录起始位点和转录变异体,构建了2个含有长度分别为2.5和1.2 kb的NFBD1启动子序列的荧光素酶报告基因重组体以及2个相应的含有外源增强子的NFBD1启动子荧光素酶报告基因重组体.启动子活性分析表明,该启动子区域具有启动子活性,且能被外源增强子有效驱动,是一个新的人NFBD1启动子.转录因子结合位点预测分析表明,该启动子区域缺乏典型的CCAAT盒和GC盒,但含有TATA盒以及STAT1,MZF1和MAZ等潜在的转录因子结合位点.结论:鉴定了一个新的NFBD1启动子.
- [1]Mitsuru, Nakanishi.[Regulation of p53 dependent apoptosis by functional binding of NFBD1/MDC1].[J].2006,81(3).
- [2]Smale, S. T..Transcription initiation from TATA-less promoters within eukaryotic protein-coding genes[J].1997,1351(1).
- [3]Lou Z,Minter Dykhouse K,Franco S,etc.MDC1 maintains genomic stability by participating in the amplification of ATM-dependent DNA damage signals.[J].2006,21(2).
- [4]Michelle, Thomas,Clodagh E, Finnegan,Katherine M-A, Rogers,etc.STAT1: a modulator of chemotherapy-induced apoptosis.[J].2004,64(22).
- [5]Michal, Goldberg,Manuel, Stucki,Jacob, Falck,etc.MDC1 is required for the intra-S-phase DNA damage checkpoint.[J].2003,421(6926).
- [6]Barry S,Latchman DS,Cragg MS,etc.STAT-1 facilitates the ATM activated checkpoint pathway following DNA damage.[J].2005,118(Pt.8).
- [7]Stucki M,Jackson SP.MDC1/NFBD1: a key regulator of the DNA damage response in higher eukaryotes.[J].2004,3(8).
- [8]Black AR,Azizkhan-Clifford J,Black JD.Sp1 and kruppel-like factor family of transcription factors in cell growth regulation and cancer.[J].2001,188(2).
- [9]Zhenkun, Lou,Katherine, Minter-Dykhouse,Xianglin, Wu,etc.MDC1 is coupled to activated CHK2 in mammalian DNA damage response pathways.[J].2003,421(6926).