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Apoptosis of human colon cancer cell line SW1116 induced by 2-[( 3-carboxy-1-oxoprogy1) amino ] -2-deoxy-D-glucose
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 18
- DOI:
- 10.3321/j.issn:1000-2790.2007.18.002
- Key Word:
- 2-(3-羧基-1-丙酰氨基)-2-脱氧-D-葡萄糖;细胞凋亡;结肠肿瘤;SW1116细胞
Abstract： 目的:研究D-葡萄糖衍生物2-(3-羧基-1-丙酰氨基)-2-脱氧-D-葡萄糖诱导人结肠癌细胞SW1116凋亡时,其形态学的变化.方法:选用不同浓度的2-(3-羧基-1-丙酰氨基)-2-脱氧-D-葡萄糖与人结肠癌细胞SW1116共同孵育不同时间后,采用倒置相差显微镜,荧光显微镜、透射电子显微镜观察人结肠癌细胞SW1116的形态结构变化.结果:经2-(3-羧基-1-丙酰氨基)-2-脱氧-D-葡萄糖的不同浓度作用24～96h后,观察发现:人结肠癌细胞SW1116出现体积缩小,荧光染色增强,胞核或胞质中可见致密浓染的颗粒状黄绿色荧光染色.细胞染色质固缩,积聚在核膜边缘呈新月状,内质网疏松并与胞膜融合形成空泡,并呈一定的浓度、时间依赖性.结论:2-(3-羧基-1-丙酰氨基)-2-脱氧-D-葡萄糖在体外具有诱导人结肠癌细胞SW1116凋亡的作用.
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