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Neuroprotecfion of remote preconditioning via activation of mitochondrial ATP-sensitive potassium channel after focal cerebral ischemia in rats
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 18
- DOI:
- 10.3321/j.issn:1000-2790.2007.18.001
- Key Word:
- 远程预处理;线粒体敏感性钾离子通道;脑缺血;再灌注
Abstract： 目的:探讨线粒体ATP敏感性钾离子通道在远程预处理对大鼠脑保护效应中的作用.方法:SD雄性大鼠,随机分为4组(每组n=10):① RPC+NS组,行远程预处理前15 min,给予生理盐水1 mL静脉注射,远程预处理1 h后行MCAO;② RPC+5-HD组,行远程预处理前15 min给予5-羟基葵酸盐(5-HD)10 mg/kg静脉注射,远程预处理后1 h行MCAO;③ DIAZ组,MCAO前30 min给予二氮嗪(DIAZ)5mg/kg腹腔注射;④ DMSO组,MCAO前30 min给予5 g/LDMSO.所有大鼠行MCAO模型阻闭120 min恢复再灌注,观察再灌注后24 h时神经功能损害并取大脑行TTC染色测量脑梗死容积百分比.结果:①神经功能障碍评分:再灌注24h后神经功能障碍评分,RPC+NS组和DIAZ组与RPC+5-HD组和DMSO组相比有统计学差异(P＜0.05),RPC+5-HD组和DMSO组相比无统计学差异(P＞0.05).②脑梗死容积百分比:再灌注24 h后脑梗死容积百分比RPC+NS组[(16.3±2.9)%,P=0.00]和DIAZ组[(17.5±8.9)%,P=0.00]明显小于RPC+5-HD组(46.1±10.1)%和DMSO组(36.4±10.9)%,而DMSO组和RPC+5-HD组相比较无统计学差异(P=0.216),RPC+NS组和DIAZ组相比较无统计学差异(P=0.747).结论:线粒体敏感性钾离子通道阻断剂可阻断远程预处理保护作用,提示线粒体敏感性钾离子通道参与远程预处理对大鼠脑缺血耐受的形成机制.
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