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Labeling of myoblasts with superparamagnetic iron oxide nanoparticle
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 17
- DOI:
- 10.3321/j.issn:1000-2790.2007.17.005
- Key Word:
- 成肌细胞;超顺磁性氧化铁;磁共振成像
Abstract： 目的:使用超顺磁性氧化铁纳米粒子(SPIO)对成肌细胞进行体外标记,探讨SPIO标记方法及SPIO对细胞的影响.方法: 常规方法进行成肌细胞培养,应用脂质体包被的SPIO标记成肌细胞,用光镜及电镜观察标记效率及对细胞增殖的影响,台盼蓝染色及JC-1观测SPIO对细胞活力的影响.结果: 普鲁士蓝染色证实了每个标记细胞胞质内有多少不等的蓝染铁颗粒,单纯SPIO标记有效率为50%,脂质体包被的SPIO标记有效率为100%,电镜也观测到细胞内的SPIO颗粒.SPIO标记对细胞无毒性,不影响细胞的增殖及活性.结论: 脂质体包被SPIO标记成肌细胞方法简单、高效、无毒,可用于磁共振对标记细胞进行活体内外无创动态示踪研究.
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