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Expression of siRNA vector against HER2 in the human breast carcinoma SKBr-3 cells
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 15
- DOI:
- 10.3321/j.issn:1000-2790.2007.15.004
- Key Word:
- HER2;RNA干涉;基因治疗
Abstract： 目的:探讨针对HER2分子的RNA干涉对乳腺癌细胞生长的影响.方法:人工合成DNA,经退火磷酸化后克隆入pSUPER载体.基因转染后通过观察细胞形态和细胞计数等方法验证发夹状小干涉RNA(siRNA)对细胞生长状态的影响,以激光共聚焦检测基因表达情况.结果:siRNA表达载体转染后可以引起SKBr-3细胞大量死亡,间接免疫荧光检测出HER2蛋白表达降低.结论:针对HER2进行RNA干涉可在体外抑制SKBr-3细胞生长.
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