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RNA interferes the expression of VEGF gene in hepatocarcinoma Hep-3B cells in vitro
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 15
- DOI:
- 10.3321/j.issn:1000-2790.2007.15.002
- Key Word:
- RNAi;siRNA;血管内皮生长因子;肝癌细胞
Abstract： 目的:通过自主构建的RNAi载体和体外合成siRNA的方法,寻找能有效干扰VEGF基因表达的载体和siRNA片段治疗肿瘤.方法:将自主构建的pcDUVEGF-1和pcDUVEGF-2,以及体外合成的Twvegf-1和Twvegf-2 siRNA片段转染肝癌Hep-3B细胞,通过RT-PCR,Western Blot和ELISA检测转染前后VEGF的mRNA及蛋白表达水平,以了解RNA干扰效果.结果:两种RNA干扰的方法均能抑制VEGF基因的表达且差异具有显著性(mRNA,0.28,0.47,0.76,0.58 vs 1.2, P＜0.01;蛋白质,138, 121, 249, 227 vs 389 μg/L, P＜0.01).结论:两个质粒及siRNA片段均能有效的抑制VEGF基因的表达.
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