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Prediction of B cell epitopes and HLA restricted CTL epitopes of human cervical cancer oncogene protein
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 14
- DOI:
- 10.3321/j.issn:1000-2790.2007.14.004
- Key Word:
- 宫颈肿瘤;癌基因蛋白质类;表位,B淋巴细胞;CTL表位
Abstract： 目的:预测人宫颈癌基因(human cervical cancer oncogene,HCCR)蛋白的二级结构,B细胞表位及其HLA-A,B限制性细胞毒性T细胞表位.方法:综合分析二级结构、亲水性、柔韧性、表面可及性与抗原性指数,预测HCCR蛋白的B细胞抗原表位;利用BIMAS,SYFPEITHI和NetCTL方法预测分析其HLA-A*0201限制性CTL表位,运用NetCTL方法对HLA-A的其他等位基因和HLA-B限制性CTL表位进行预测分析.结果:HCCR蛋白的二级结构主要由α-螺旋结构组成,B细胞优势表位位于N端第41～53,216～228,310～325和355～360区段;预测得到5个HLA-A*0201限制性CTL优势表位分别为YLVFLLMYL(152～160),YLFPRQLLI(159～167),LLLHNVVLL(343～351),CLFLGIISI(138～146)和SIPPFANYL(145～153),HCCR蛋白HLA-A,B限制CTL表位主要位于胞外区.结论:应用多参数预测HCCR蛋白B细胞表位及其HLA-A,B限制性细胞毒性T细胞表位,为进一步实验鉴定其表位进而制备单克隆抗体和基于HCCR抗原的肿瘤免疫学治疗奠定了基础.
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