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Effect of polycaprolactone-based electrospun nanofibrous framework on proliferation and differentiation of bone marrow stromal cells in vitro
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 13
- DOI:
- 10.3321/j.issn:1000-2790.2007.13.001
- Key Word:
- 电纺纤维;聚己内酯;骨髓基质细胞
Abstract： 目的:观察聚己内酯(PCL)电纺纤维支架材料对骨髓基质细胞(BMSCs)增殖及分化特点的影响,评价其作为骨组织工程的支架材料的应用前景.方法:将兔BMSCs与PCL电纺纤维支架材料在培养板内共培养.采用形态学观察、MTT法及ALP检测等方法检测BMSCs在PCL电纺纤维表面的粘附、增殖和分化能力.结果:体外培养的BMSCs能在PCL电纺纤维表面正常粘附和增殖,并且表达较高的碱性磷酸酶活性.结论:PCL电纺纤维适合作为支架材料应用于BMSCs为种子细胞的组织构建.
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