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Expression and role of MAPK signaling pathway in hyperoxia-induced lung injury
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 12
- DOI:
- 10.3321/j.issn:1000-2790.2007.12.002
- Key Word:
- 高氧症;急性病;肺/损伤;MAP激酶信号系统;ROS
Abstract： 目的:探讨丝裂原活化蛋白激酶(MAPK)信号通路三亚族ERK,p38,JNK在大鼠高氧性肺损伤动物模型中的表达及作用.方法:24只3周龄Wistar大鼠随机分成4组:空气对照组和高氧暴露3,7和14 d组,每组动物6只.高氧暴露组置于常压高氧仓中(O2≥95%),空气对照组置于常压空气中(O2=21%).光镜下观察肺组织病理学改变,采用二步法免疫组化检测磷酸化ERK,p38,JNK在肺组织中的分布.Western Blot检测磷酸化MAPK蛋白表达变化.结果:高氧暴露3,7 d组出现急性肺损伤的典型病理学特征,高氧暴露14 d组肺间质及纤维细胞增生明显.免疫组化显示空气对照组仅肺泡上皮细胞及气道上皮细胞见少量磷酸化ERK,p38,JNK阳性表达,高氧暴露组则阳性细胞明显增多,广泛分布于肺内细胞中:肺泡上皮细胞、气道上皮细胞、胸膜间皮细胞、浸润炎细胞及间质纤维细胞,p38阳性表达尤多见于炎性细胞中.Western Blot显示高氧暴露组较空气对照组磷酸化蛋白表达明显增强,ERK,JNK高氧暴露7 d组表达最强(P＜0.05),p38在高氧暴露14 d组表达最为明显(P＜0.05).结论:高浓度氧可激活MAPK信号途径,磷酸化ERK,p38,JNK在高氧损伤肺组织表达明显增加,MAPK三亚族ERK,p38,JNK活性变化在时间上并不具有同步性.
- [1]张艰,李圣青,戚好文,等.p38 MAPK在大鼠急性肺损伤模型中的表达及抗氧化剂NAC的影响[J].2004,(15).
- [2]Nash SP,Heuertz RM.Blockade of p38 map kinase inhibits complement-induced acute lung injury in a murine model.[J].2005,5(13/14).
- [3]Lee PJ,Ma B,Lee CG,etc.ERK1/2 mitogen-activated protein kinase selectively mediates IL-13-induced lung inflammation and remodeling in vivo.[J].2006,116(1).
- [4]Xuchen, Zhang,Peiying, Shan,Madhu, Sasidhar,etc.Reactive oxygen species and extracellular signal-regulated kinase 1/2 mitogen-activated protein kinase mediate hyperoxia-induced cell death in lung epithelium.[J].2003,28(3).
- [5]Liu Y,Fan X,Wang Q,etc.P38 mitogen-activated protein kinase inhibition attenuates pulmonary inflammatory response in a rat cardiopulmonary bypass model.[J].2006,30(1).
- [6]Buckley S,Warburton D,Barsky L,etc.In vivo inosine protects alveolar epithelial type 2 cells against hyperoxia-induced DNA damage through MAP kinase signaling.[J].2005,288(3).
- [7]Kujime K,Gon Y,Shimizu K,etc.p38 mitogen-activated protein kinase and c-jun-NH2-terminal kinase regulate RANTES production by influenza virus-infected human bronchial epithelial cells.[J].2000,164(6).
- [8]Min-Joon Han,An-Sik Chung,Bu-Yeo Kim,etc.Cell Proliferation Induced by Reactive Oxygen Species Is Mediated via Mitogen-activated Protein Kinase in Chinese Hamster Lung Fibroblast (V79) Cells[J].2003,15(1).
- [9]Reddy SP,Mossman BT,Lounsbury KM.Oxidants and signaling by mitogen-activated protein kinases in lung epithelium.[J].2006,34(6).
- [10]Gong ZN,Xu GL,Yao L,etc.Attenuation of acute lung injury in mice by oxymatrine is associated with inhibition of phosphorylated p38 mitogen-activated protein kinase.[J].2005,98(1).
- [11]Carvalho H,Gonzalez Flecha B,Evelson P,etc.Mitogen-activated protein kinases modulate H2O2-induced apoptosis in primary rat alveolar epithelial cells.[J].2004,92(3).