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Expression and significance of GAP-43 in hippocampal CA1 region after cerebral ischemic preconditioning in neonatal rats
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 5
- DOI:
- 10.3321/j.issn:1000-2790.2007.05.003
- Key Word:
- 脑缺血;缺血预处理;生长相关蛋白43;海马;Wistar大鼠;新生
Abstract： 目的:观察新生Wistar鼠脑缺血再灌注后海马CA1区生长相关蛋白43(GAP-43)的表达和意义. 方法:通过阻断7日龄新生Wistar大鼠右侧颈总动脉45 min制备脑缺血模型,设置假手术组、缺血再灌注组和预缺血-缺血再灌注组. 采用免疫组化方法和计算机图像分析技术检测三组新生鼠不同时点海马CA1区脑组织GAP-43的动态变化及三组光镜下脑组织病理改变. 结果: ①脑组织病理改变:假手术组大鼠无异常病理改变,缺血再灌注组神经细胞变性、坏死,预缺血组-病理损伤较缺血再灌注组轻. ②GAP-43表达:缺血再灌注组海马GAP-43表达在再灌注后24 h时开始增高,7 d达高峰,至14 d与假手术组比较差异有统计学意义(P＜0.05);预缺血-缺血再灌注组GAP-43表达较缺血再灌注组增加更明显,与假手术组和缺血再灌注组比较均差异有统计学意义(P＜0.05). 结论: 新生鼠脑缺血预处理可减轻再次严重脑缺血对神经细胞的损伤,脑缺血预处理后海马CA1区GAP-43表达和合成增加,GAP-43表达增加可能与神经元再生和轴突重塑有关,是脑缺血后神经细胞内源性代偿机制之一.
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