You Position: Home > Paper

Establishment of culture system for mouse lymphatic endothelial cells adhered to rat-tail collagen

( views:128, downloads:4 )
Author:
No author available
Journal Title:
JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
Issue:
5
DOI:
10.3321/j.issn:1000-2790.2007.05.002
Key Word:
鼠尾胶;贴黏剂;内皮,淋巴管;细胞培养

Abstract: 目的:建立一个简便、廉价和稳定的小鼠淋巴管内皮细胞(LEC)培养体系. 方法:应用不完全弗氏佐剂诱导小鼠腹腔淋巴管瘤形成,消化法分离获得LEC,置于自制的鼠尾胶包被的培养瓶(板)中培养. 以免疫荧光化学法检测LEC特异性标记物VEGFR-3和LYVE-1的表达,以3H-TdR掺入率测定和细胞倍增时间检测LEC的增殖活力,以淋巴管形成试验判定LEC能否形成淋巴管样结构. 结果:不完全弗氏佐剂能诱导小鼠腹腔淋巴管瘤形成,所获LEC活细胞数大于98%. 免疫荧光化学检测表明, LEC表达VEGFR-3和LYVE-1. 在鼠尾胶包被的培养瓶(板)中, LEC生长状况良好, 3H-TdR掺入率明显增加,倍增时间为(42.7±3.2) h;在鼠尾胶凝胶中,LEC能形成淋巴管样结构. 结论:鼠尾胶为贴黏剂的体系是一种简便、廉价和稳定的小鼠LEC培养体系.

WanfangData CO.,Ltd All Rights Reserved
About WanfangData | Contact US
Healthcare Department, Fuxing Road NO.15, Haidian District Beijing, 100038 P.R.China
Tel:+86-010-58882616 Fax:+86-010-58882615 Email:yiyao@wanfangdata.com.cn