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Construction of siRNA eukaryotic expression vectors of PEG10 gene and their apoptosis-inducing effect on transfected HepG2 cells
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 3
- DOI:
- 10.3321/j.issn:1000-2790.2007.03.004
- Key Word:
- PEG10基因;RNA干扰;表达载体
Abstract： 目的:构建针对遗传印记基因PEG10的siRNA真核表达载体,体外观察其对肝癌HepG2细胞凋亡的影响. 方法:设计针对PEG10基因的3个siRNA靶序列,构建真核表达载体siRNA1,siRNA2和siRNA3,脂质体分别转染HepG2细胞后实时定量PCR,检测其对HepG2细胞PEG10基因表达的影响,MTT法、流式细胞仪和激光共聚焦检测转染siRNA后的HepG2细胞的生长活性及凋亡. 结果:成功构建了针对PEG10基因的3个特异性siRNA表达载体. 它们不同程度地抑制了HepG2细胞PEG10基因的表达,以siRNA2抑制效率最高,可达93.99%. HepG2细胞的生长也明显受到抑制(P＜0.05),大量HepG2细胞凋亡,siRNA2凋亡率最高,为66.91%. 结论:靶向PEG10基因的siRNA表达载体可抑制肝癌HepG2细胞的生长,诱导细胞凋亡,该作用与降低PEG10基因的表达有关.
- [1]常莹,陶璐薇,陈孝平,等.肝癌组织中遗传印记基因PEG10表达的特异性及其意义[J].2005,(12).
- [2]V, Vella,L, Sciacca,G, Pandini,etc.The IGF system in thyroid cancer: new concepts.[J].2001,54(3).
- [3]Ono R,Ishino F,Aisaka K,etc.A retrotransposon-derived gene, PEG10, is a novel imprinted gene located on human chromosome 7q21.[J].2001,73(2).
- [4]Baldwin RM,Sanchez OH,Khokha R,etc.Transgenic overexpression of IGF-II induces spontaneous lung tumors: a model for human lung adenocarcinoma.[J].2003,22(6).
- [5]Miguel, Constancia,Myriam, Hemberger,Jennifer, Hughes,etc.Placental-specific IGF-II is a major modulator of placental and fetal growth.[J].2002,417(6892).
- [6]Colin L. Stewart,Graziella Piras,Lidia Hernandez,etc.Paternal and maternal genomes confer opposite effects on proliferation, cell-cycle length, senescence, and tumor formation[J].2003,100(23).
- [7]L, Partridge,L D, Hurst.Sex and conflict.[J].1998,281(5385).