You Position: Home > Paper
Construction and expression of eukaryotic expression vector of Mycobacterium tuberculosis Rv2389
( views:202, downloads:0 )
- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 2
- DOI:
- 10.3321/j.issn:1000-2790.2007.02.001
- Key Word:
- 分枝杆菌,结核;Rv2389;基因表达
Abstract： 目的:构建结核分枝杆菌Rv2389基因真核表达载体.方法:PCR扩增Rv2389基因,测序正确后克隆入真核表达载体pCDNA3.1(-),重组质粒酶切鉴定正确后以阳离子聚合物转染CHO细胞后, 分别以RT-PCR方法检测mRNA表达和间接免疫荧光技术检测目的蛋白的表达.结果:构建了重组质粒pCDNA- Rv2389, RT-PCR结果证明Rv2389可在CHO细胞中转录,间接免疫荧光检测证明,表达有Rv2389蛋白的细胞着染.结论:成功构建了结核分枝杆菌Rv2389基因的真核表达载体pCDNA-Rv2389,Rv2389基因可以在CHO细胞中表达.
- [1]师长宏,范雄林,徐志凯,等.融合表达Ag85B-ESAT6的结核分枝杆菌真核表达载体的构建及其免疫原性[J].2004,(2).
- [2]高雪,薛莹,姜泓,等.结核分枝杆菌furA基因片段的克隆、表达和分离纯化[J].2004,(18).
- [3]T J, Smith,S A, Blackman,S J, Foster.Peptidoglycan hydrolases of Bacillus subtilis 168.[J].1996,2(1).
- [4]Sassetti CM,Boyd DH,Rubin EJ.Genes required for mycobacterial growth defined by high density mutagenesis.[J].2003,48(1).
- [5]Kaprelyants AS,Mukamolova GV,Young DI,etc.A family of autocrine growth factors in Mycobacterium tuberculosis.[J].2002,46(3).
- [6]Malhotra D,Bamezai RN,Kumar H,etc.How far have we reached in tuberculosis vaccine development?[J].2003,29(4).
- [7]Kaprelyants AS,Young DI,Young M,etc.A bacterial cytokine.[J].1998,95(15).
- [8]Bolhuis A,Tjalsma H,Bron S,etc.Signal peptide-dependent protein transport in Bacillus subtilis: a genome-based survey of the secretome.[J].2000,64(3).
- [9]Cohen-Gonsaud M,Keep NH,Ward J,etc.Resuscitation-promoting factors possess a lysozyme-like domain[J].2004,29(1).