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Rapid elevation of intracellular free calcium concentration in astrocytes after treated by glucocorticoid
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 23
- DOI:
- 10.3321/j.issn:1000-2790.2006.23.004
- Key Word:
- 应激;糖皮质激素;星形细胞;钙
Abstract： 目的:研究应激水平的糖皮质激素(GC)在早期快速效应中对星形胶质细胞内游离Ca2+浓度([Ca2+]i)的影响及作用机制. 方法:原代培养新生Wistar大鼠海马星形胶质细胞,用1 μmol/L的皮质酮(CORT)处理星形胶质细胞,激光共聚焦显微镜下观察CORT快速作用致星形胶质细胞内游离[Ca2+]i的变化,并设立N-甲基-D-天冬氨酸(NMDA),受体拮抗剂5-甲基二氢丙环庚烯亚胺马来酸(MK-801),牛血清白蛋白偶联CORT (BSA-CORT)及无细胞外钙等干预组,进一步检测[Ca2+]i变化. 结果:与未处理对照细胞(141.2±4.7)相比,CORT可快速介导星形胶质细胞内荧光强度增加(177.1±3.8, P＜0.01),但细胞外钙为零时无明显变化(148.5±6.3);NMDA受体激活可使荧光强度进一步增强(195.3±7.2,P＜0.01),NMDA受体拮抗剂MK-801可部分拮抗荧光强度升高(186.4±1.8);BSA-CORT可产生与CORT类似的效应(200.7±12.2,P＜0.01). 结论:应激水平的GC在早期可快速增加星形胶质细胞胞内[Ca2+]i,激活NMDA受体是其中的重要机制之一.
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