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Inhibitory effects of genistein on proliferation in human ovarian carcinoma cell line 3AO and related mechanism
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 23
- DOI:
- 10.3321/j.issn:1000-2790.2006.23.003
- Key Word:
- 染料木黄酮;卵巢肿瘤;细胞系;细胞凋亡
Abstract： 目的:研究植物雌激素染料木黄酮对人卵巢癌细胞系3AO细胞增殖的抑制作用和凋亡的诱导作用,探讨其抗癌作用的机制. 方法:应用MTT法检测不同浓度染料木黄酮对3AO细胞的生长抑制作用,电镜观察药物作用后细胞的超微结构改变,TUNEL法确定凋亡,流式细胞仪检测细胞周期、定量分析凋亡,免疫细胞化学技术检测雌激素β受体的表达. 结果: 3AO细胞经不同浓度染料木黄酮处理后,细胞生长明显受到抑制,且这种抑制作用随时间延长及浓度增高而增强,10 mg/L和20 mg/L染料木黄酮作用72 h后,抑制率分别达到54.24%和65.99%. 染料木黄酮阻断3AO细胞生长于G2/M期,在G0/G1前出现典型亚二倍体凋亡峰,且凋亡呈时间依赖性. 电镜观察到用药后凋亡细胞典型的形态学特征. TUNEL法观察到大量阳性凋亡细胞. 免疫细胞化学法检测到用药后,ERβ表达明显加强. 结论:染料木黄酮对3AO细胞的生长有明显的呈时间及浓度依赖性的抑制作用,并诱导其发生凋亡. 染料木黄酮可能通过雌激素β受体途径发挥药理作用,诱导卵巢癌细胞发生凋亡.
- [1]黄艳红,辛晓燕,陈亚琼.染料木素与17β雌二醇对去势大鼠生殖系统的影响[J].2004,(6).
- [2]李昱,米粲.Genistein对人卵巢癌细胞系3AO抑制增殖和促进凋亡的作用及机制探讨[J].2003,(6).
- [3]金伟,马力.染料木黄酮对顺铂诱导肝癌SMMC-7721细胞凋亡的增敏作用[J].2005,(8).
- [4]K, Griffiths,M S, Morton,L, Denis.Certain aspects of molecular endocrinology that relate to the influence of dietary factors on the pathogenesis of prostate cancer.[J].1999,35.
- [5]X, Chen,J J, Anderson.Isoflavones inhibit proliferation of ovarian cancer cells in vitro via an estrogen receptor-dependent pathway.[J].2001,41(1-2).
- [6]Dvorak HF.Vascular permeability factor/vascular endothelial growth factor: a critical cytokine in tumor angiogenesis and a potential target for diagnosis and therapy.[J].2002,20(21).
- [7]Yanaida Y,Kohno H,Tanaka T,etc.Inhibitory effects of estrogenic compounds, 4-nonylphenol and genistein, on 7,12-dimethylbenz(a)anthracene-induced ovarian carcinogenesis in rats.[J].2002,52(1).
- [8]Z M, Shao,J, Wu,Z Z, Shen,etc.Genistein exerts multiple suppressive effects on human breast carcinoma cells.[J].1998,58.
- [9]T, Rutherford,W D, Brown,E, Sapi,etc.Absence of estrogen receptor-beta expression in metastatic ovarian cancer.[J].2000,96(3).