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Establishment of model of Plasmodium berghei transfected with Plasmodium falciparum apical membrane antigen-1 gene
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 19
- DOI:
- 10.3321/j.issn:1000-2790.2006.19.005
- Key Word:
- 伯氏疟原虫;恶性疟原虫;顶端膜抗原1;转染
Abstract： 目的: 建立恶性疟原虫顶端膜抗原1(AMA-1)基因转染伯氏疟原虫的模型. 方法: 将含有恶性疟原虫AMA-1基因的重组转染质粒pDB.DTm.DB./AB.AF.DB.用BglI酶切线性化. 伯氏疟原虫经体外培养和分离后进行电转化,转化的原虫经药物筛选后进行PCR检测. 结果: PCR检测显示转染伯氏疟原虫存在恶性疟原虫AMA-1基因. 结论: 成功建立了恶性疟原虫AMA-1基因转染伯氏疟原虫的模型,为进一步研究提供了基础.
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