You Position: Home > Paper
Expression of early muscle specific mRNA in bone marrow stem cells
( views:164, downloads:0 )
- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 18
- DOI:
- 10.3321/j.issn:1000-2790.2006.18.002
- Key Word:
- 骨髓祖代细胞;组织特异性;肌形成调节因子
Abstract： 目的:研究早期肌肉特异性mRNA在骨髓干细胞中的特异性表达.方法:利用RT-PCR研究不同时期C57鼠和不同代龄SD大鼠骨髓干细胞早期肌肉特异性mRNA(包括MyoD,Myf5,myogenin,MRF4和desmin)的表达.结果:Myf5及desmin在新鲜分离、培养了2 d和7 d的悬浮与贴壁的C57小鼠骨髓干细胞中均有表达,没有检测到MyoD,myogenin和MRF4在此时期小鼠骨髓干细胞的表达.MyoD与desmin在原代至第6代SD大鼠骨髓间质干细胞中均有表达,没有检测到myogenin和MRF4在各代细胞中的表达.结论:骨髓干细胞能表达一定水平的早期肌肉特异性mRNA.
- [1]栗艳,闫露,沈敏.地塞米松诱导促进BMP-2基因转染的兔MSCs的成骨转化[J].2005,(6).
- [2]张为西,张成,刘焯霖,等.Duchenne肌营养不良模型鼠骨髓移植后dystrophin的表达[J].2002,(6).
- [3]康新勤,臧伟进,胥晓丽,等.大鼠骨髓间充质干细胞体外分化特性[J].2004,(14).
- [4]Manabu, Inobe,Ikuko, Inobe,Gregory R, Adams,etc.Effects of microgravity on myogenic factor expressions during postnatal development of rat skeletal muscle.[J].2002,92.
- [5]E, Gussoni,Y, Soneoka,C D, Strickland,etc.Dystrophin expression in the mdx mouse restored by stem cell transplantation.[J].1999,401(6751).
- [6]M F, Pittenger,A M, Mackay,S C, Beck,etc.Multilineage potential of adult human mesenchymal stem cells.[J].1999,284.
- [7]Janowska Wieczorek A,Kucia M,Reca R,etc.Stem cell plasticity revisited: CXCR4-positive cells expressing mRNA for early muscle, liver and neural cells 'hide out' in the bone marrow.[J].2004,18(1).
- [8]L A, Sabourin,M A, Rudnicki.The molecular regulation of myogenesis.[J].2000,57(1).