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Analysis of different protein expressions in different liver cell strains in vitro using SELDI
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 16
- DOI:
- 10.3321/j.issn:1000-2790.2006.16.001
- Key Word:
- 表面增强激光解吸/离子化/飞行时间质谱;蛋白质陈列分析;肝肿瘤;肿瘤细胞,培养的;差异蛋白
Abstract： 目的: 运用表面增强激光解吸/离子化/飞行时间质谱(SELDI-TOF-MS) 技术,检测体外培养的肝癌细胞株(HepG2),转染乙肝病毒的肝癌细胞株(HepG2.2.15)与正常肝细胞株(LO2)蛋白质的差异表达,筛选肝细胞癌的标志蛋白,为进一步研究肝癌发病的蛋白质组学机制奠定基础. 方法: 常规培养上述3种细胞,细胞状态良好时收集细胞,裂解细胞后采用 SELDI-TOF-MS技术用WCX2芯片检测HepG2, HepG2.2.15, LO2细胞内蛋白质组学的差异表达. 结果: WCX2芯片共捕获91个蛋白,在Mr 5000～15 000区段,与正常肝细胞株比较,有7个蛋白质在两种肝癌细胞中出现明显变化,其中2个蛋白在肝癌细胞中表达量增高,5个蛋白在肝癌细胞中表达量降低;另外两种肝癌细胞株也有其各自的标志蛋白,9个蛋白分子在HepG2表达量增高,10个蛋白分子在HepG2.2.15表达量增高. 结论: SELDI蛋白芯片技术检测可作为检测肝癌早期生物标记的方法,它简便,敏感性高,重复性好,本文发现的这些组织特异性蛋白生物标记对肝癌的早期诊断有潜在的应用价值,对我们从血清或组织标本中筛选和鉴定不同型别肝癌细胞之间的标志蛋白有重要意义,从而为从蛋白质水平研究肝癌的发病机制及新的治疗靶位的寻找奠定了一定的基础.
- [1]夏梁,蔡伟丽,张丽娟,等.体外培养的不同亚型肺癌细胞株差异蛋白的初步分析[J].2004,(1).
- [2]丁守怡,钱冬萌,闫志勇,等.蛋白质芯片飞行质谱技术检测体外培养的肝癌细胞株与转染HBV的肝癌细胞株蛋白质的差异表达[J].2005,(14).
- [3]F, von Eggeling,H, Davies,L, Lomas,etc.Tissue-specific microdissection coupled with ProteinChip array technologies: applications in cancer research.[J].2000,29(5).
- [4]Stoop AA,Jespers L,Eldering E,etc.High-density mutagenesis by combined DNA shuffling and phage display to assign essential amino acid residues in protein-protein interactions: application to study structure-function of plasminogen activation inhibitor 1 (PAI-I).[J].2000,301(5).
- [5]L A, Liotta,E C, Kohn,E F, Petricoin.Clinical proteomics: personalized molecular medicine.[J].2001,286(18).
- [6]Kerjan P.,Mirande M.,Robinson JC..Macromolecular assemblage of aminoacyl-tRNA synthetases: Quantitative analysis of protein-protein interactions and mechanism of complex assembly[J].2000,304(5).
- [7]J, Peng,S P, Gygi.Proteomics: the move to mixtures.[J].2001,36(10).
- [8]N, Johnsson,P, Nguyen Van,H D, S?ling,etc.Functionally distinct serine phosphorylation sites of p36, the cellular substrate of retroviral protein kinase; differential inhibition of reassociation with p11.[J].1986,5(13).