Effects of interleukin-10 on proliferation and p27 expression in cultured cardiac fibroblasts induced by arginine vasopressin
- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 14
- DOI:
- 10.3321/j.issn:1000-2790.2006.14.003
- Key Word:
- 白细胞介素10;精氨酸升压素;心肌;成纤维细胞;p27蛋白
Abstract： 目的:研究白细胞介素10(IL-10)对血管升压素(AVP)诱导大鼠心脏成纤维细胞(CFs)增殖及p27蛋白表达的影响. 方法:以培养的新生SD大鼠CFs为实验模型,四氮唑盐(MTT)比色法检测CFs增殖,流式细胞分析仪(FCM)技术测定细胞周期及p27蛋白表达阳性率. 结果:① 10-7 mol/L AVP作用24 h,CFs的A490为0.216±0.013,较空白组(0.132±0.006)明显增高(P＜0.01). ② 10-11～10-8 kg/L IL-10+10-7 mol/L AVP组A490分别为0.201±0.007,0.187±0.006,0.173±0.010,0.157±0.029,均明显低于10-7 mol/L AVP组(其中 10-11组P＜0.05,10-10,10-9,10-8三组P均 ＜0.01). ③10-7 mol/L AVP作用24 h,CFs的S期百分率(12.3±0.7)及增殖指数(19.6±0.9)较空白组(4.2±0.5, 7.1±0.6)均明显增高(P＜0.01);10-9 kg/L IL-10+10-7 mol/L AVP组S期百分率(9.6±1.1)及增殖指数(13.86±1.28)均明显低于AVP组(P＜0.01). ④ 10-7 mol/L AVP作用24 h,p27蛋白表达阳性率为(63.7±2.4)%,较空白组(78.5±2.5)%明显降低(P＜0.01);10-9 kg/L IL-10+10-7 mol/L AVP组p27蛋白表达阳性率为(70.5±2.4)%,较AVP组(63.7±2.4)%明显增高(P＜0.01). 结论:IL-10具有抑制AVP诱导CFs增殖的作用,p27蛋白可能是IL-10调控AVP诱导CFs增殖的细胞内靶蛋白.
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