You Position: Home > Paper
Establishment and biological charac-terization of an immortalized human fetal hepatocyte line
( views:205, downloads:7 )
- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 11
- DOI:
- 10.3321/j.issn:1000-2790.2006.11.004
- Key Word:
- 永生化;肝细胞;细胞系;生物学特性;SV40;脂质体
Abstract： 目的:构建永生化肝细胞系并对其生物学特性及某些功能进行研究. 方法:利用SV40T基因和真核表达载体pcDNA3.1经脂质体转染至体外分离培养的人胚胎肝细胞,使其永生化,进一步鉴定其形态学特征和生物学功能. 结果:经G418 700～300 mg/L筛选,40 d后获得一株阳性克隆. 形态学观察发现,该细胞具有原代培养肝细胞的大多数典型特征. 永生化胚胎肝细胞克隆形成率为31.2%,染色体核型分析表明细胞核型无明显异常,软琼脂集落形成试验表明细胞在软琼脂中不能生长,免疫组化证明SV40T基因已整合入细胞,而且该细胞具有合成白蛋白的功能. 结论:新建胚胎肝细胞系具有与原代肝细胞类似的形态特征及生物学功能,可以成为生物人工肝及肝细胞移植研究中的理想细胞材料.
- [1]Berg A,Bodusch K,Denner J,etc.Sensitive and specific immunological detection methods for porcine endogenous retroviruses applicable to experimental and clinical xenotransplantation.[J].2001,8(2).
- [2]K, Leckel,R A, Blaheta,B H, Markus.[State of hepatocyte transplantation: a risk or a chance?].[J].2003,128(4).
- [3]Hodgson H.Liver cells: biology to therapeutics.[J].2003,3(2).
- [4]Shirota C,Nagata H,Ito M,etc.Route of hepatocyte delivery affects hepatocyte engraftment in the spleen.[J].2003,76(4).
- [5]C, Di Campli,M, Nestola,A C, Piscaglia,etc.Cell-based therapy for liver diseases.[J].1900,7(2).
- [6]Lisheng Wang,Junhong Sun,Li Li,etc.Comparison of porcine hepatocytes with human hepatoma (C3A) cells for use in a bioartificial liver support system[J].1998,7(5).
- [7]Naoya, Kobayashi,Teru, Okitsu,Noriaki, Tanaka.Cell choice for bioartificial livers.[J].2003,52(3).