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Role of sodium selenite in regulating expressions of p38MAPK and MCP-1 in rat mesangial cells
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 11
- DOI:
- 10.3321/j.issn:1000-2790.2006.11.002
- Key Word:
- 亚硒酸钠;p38丝裂原活化蛋白激酶;单核细胞趋化蛋白-1;系膜细胞;糖尿病肾病
Abstract： 目的:观察亚硒酸钠对大鼠肾小球系膜细胞系HBZY-1表达p38丝裂原活化蛋白激酶(p38MAPK)和单核细胞趋化蛋白-1 (MCP-1)的影响,从而研究硒在防治糖尿病肾病(DN)中的作用机制. 方法:分别以高葡萄糖、高胰岛素、过氧化氢和糖基化终末产物(AGEs)刺激HBZY-1细胞;先给予100 nmol/L亚硒酸钠预处理后,再分别以上述4种刺激因素孵育HBZY-1细胞,分别检测HBZY-1细胞p38MAPK和MCP-1的表达并比较. 结果:4种刺激因素均可作为独立因素,导致HBZY-1细胞p38MAPK和MCP-1表达量增加;亚硒酸钠能抑制上述4种因素所致的p38MAPK和MCP-1的表达. 结论:亚硒酸钠通过抑制p38MAPK和MCP-1在HBZY-1细胞的表达, 从而有效防治DN的发生发展,表明硒在DN的防治过程中发挥积极作用.
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