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Expressions of Caspase-3, TNF-α and TNFRΙ and their relationship with cell apoptosis in oral lichen planus
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 7
- DOI:
- 10.3321/j.issn:1000-2790.2006.07.002
- Key Word:
- 扁平苔癣;口腔;Caspase-3;肿瘤坏死因子;TNFRΙ;细胞凋亡
Abstract： 目的: 探讨口腔扁平苔藓(OLP)中Caspase-3, TNF-α和TNFRΙ的表达意义及其与细胞凋亡的关系. 方法: 采用脱氧核糖核苷酸末端转移酶介导的原位缺口末端标记法(TUNEL法)、SP免疫组化方法检测33例OLP及7例正常口腔黏膜(对照组)中Caspase-3, TNF-α和TNFRΙ的表达及细胞凋亡情况. 结果: OLP中上皮细胞凋亡指数(AI)明显高于正常对照组(P<0.05),而固有层的AI明显低于正常对照组(P<0.05). OLP的上皮层和固有层Caspase-3阳性表达明显高于正常对照组(P<0.025). OLP中上皮层TNFRI及固有层TNF-α阳性表达明显高于正常对照组(P<0.025),而上皮层TNF-α及固有层TNFRI阳性表达则明显低于正常对照组(P<0.025),糜烂型组固有层中TNF-α阳性表达明显高于非糜烂型组(P<0.05). OLP上皮层Caspase-3阳性表达与其自身AI呈正相关(r=0.631, P<0.05),而固有层淋巴细胞Caspase-3阳性表达与自身的AI无相关性(P>0.05);TNF-α/TNFRI表达与OLP上皮及固有层细胞的AI密切相关. 结论: OLP中同时存在角质细胞凋亡亢进及固有层淋巴细胞凋亡的减少,这种凋亡的异常可能与OLP的发生发展密切相关.
- [1]沈丽佳,阮萍,殷操,等.口腔扁平苔藓中肿瘤坏死因子α和肿瘤坏死因子受体I表达及与细胞凋亡的关系[J].2004,(9).
- [2]沈丽佳,阮萍,殷操,等.口腔扁平苔藓中TNF-α表达及其与肥大细胞的关系[J].2004,(6).
- [3]C, Simark-Mattsson,G, Bergenholtz,M, Jontell,etc.Distribution of interleukin-2, -4, -10, tumour necrosis factor-alpha and transforming growth factor-beta mRNAs in oral lichen planus.[J].1999,44(6).
- [4]Ito T,Kawabe R,Hayashi H,etc.Cell proliferation activity and the expression of cell cycle regulatory proteins in oral lichen planus.[J].2002,31(4).
- [5]Gulec AI,Atasoy M,Erdem MT,etc.Increased Serum Levels of Tumor Necrosis Factor Alpha in Lichen planus.[J].2003,207(4).
- [6]A, Nakagawara,Y, Nakamura,H, Ikeda,etc.High levels of expression and nuclear localization of interleukin-1 beta converting enzyme (ICE) and CPP32 in favorable human neuroblastomas.[J].1997,57(20).