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Influences of age and duration of status convulsivus on cytochrome C release in hippocampus
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 7
- DOI:
- 10.3321/j.issn:1000-2790.2006.07.001
- Key Word:
- 惊厥;细胞色素C;年龄;海马;大鼠,Wistar
Abstract： 目的: 探讨年龄和惊厥持续时间对惊厥持续状态(SC)后海马细胞色素C(cytC)释放的影响. 方法:健康成年(ARs)和生后20 d幼龄Wistar鼠(IRs)各80只,经腹腔注射氯化锂-匹罗卡品分别诱发持续惊厥发作30 min的SC (30 min SC)和3 h SC,在SC后3 h至7 d的6个不同时点上处死动物,采用流式细胞仪检测海马细胞cytC含量,比较两组间的动态变化. 结果:① 30 min SC后3 h,两组大鼠海马细胞cytC含量均升高,6 h达到高峰,12 h后开始回落. 两组cytC含量峰值分别为32.47±6.28,33.23±8.66,显著高于对照组(P<0.01). ②30 min SC后7 d时,IRs组cytC含量已降至正常,而ARs组仍高于正常对照组(P<0.05). 在SC后1, 3, 7 d, IRs组海马细胞cytC含量均显著低于ARs组. ③3 h SC后3, 6 h,两组海马细胞cytC含量均显著高于30 min SC后的相同观察时点(P<0.05). 经偏相关参数分析,在排除年龄的影响后,不同惊厥持续时间与海马细胞cytC含量呈显著正相关(r=0.66,P<0.05). 结论:①严重惊厥发作可导致海马细胞cytC的释放. ②年龄和惊厥持续时间均是影响惊厥后海马细胞cytC释放的重要因素. ③惊厥持续时间越长,海马细胞cytC释放越明显. ④与成年鼠不同,幼年鼠海马细胞cytC释放启动后不久即迅速回落,提示幼年脑内可能存在一个主动抑制海马细胞cytC释放的保护性反应.
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