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Construction and identification of a Mycobacterium smegmatis strain to targetedly deliver eukaryotic coexpression plasmid encoding human granulysin and murine single chain IL-12
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 5
- DOI:
- 10.3321/j.issn:1000-2790.2006.05.004
- Key Word:
- 颗粒溶素;白细胞介素12;耻垢分枝杆菌;穿梭质粒;巨噬细胞
Abstract： 目的:构建携带编码人天然颗粒溶素(GLS)和小鼠单链IL-12基因的真核共表达载体pZM03,并构建可将其靶向递送入巨噬细胞中进行表达的重组耻垢分枝杆菌菌苗.方法:将人GLS,小鼠单链IL-12基因和分枝杆菌复制子OriM同时克隆进多启动子真核共表达载体pBudCE4.1中,得到穿梭质粒pZM03.以pZM03电转化耻垢分枝杆菌mc2-155,通过PCR方法检测重组菌中携带的真核共表达质粒;通过与小鼠巨噬细胞株RAW264.7共孵育检测重组菌的靶向性.结果:成功得到了可靶向递送GLS/IL-12真核共表达质粒pZM03进入巨噬细胞的新型重组菌.结论:利用耻垢分枝杆菌作为减毒生物体载体向巨噬细胞中靶向递送真核表达质粒是可能的.
- [1]江山,朱道银,蒋英,等.结核分枝杆菌Ag85B-Ag85A双抗原融合真核表达质粒的构建及表达[J].2003,(21).
- [2]骆旭东,朱道银,陈全,等.结核分枝杆菌Ag85B和MPT64基因疫苗的免疫原性比较[J].2003,(17).
- [3]江山,朱道银,骆旭东,等.结核分枝杆菌Ag85B与IL-12基因真核共表达质粒的构建及表达[J].2004,(6).
- [4]R A, Seder,A V, Hill.Vaccines against intracellular infections requiring cellular immunity.[J].2000,406(6797).
- [5]R, Bryk,C D, Lima,H, Erdjument-Bromage,etc.Metabolic enzymes of mycobacteria linked to antioxidant defense by a thioredoxin-like protein.[J].2002,295(5557).
- [6]Ottenhoff TH,Verreck FA,Lichtenauer Kaligis EG,etc.Genetics, cytokines and human infectious disease: lessons from weakly pathogenic mycobacteria and salmonellae.[J].2002,32(1).