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Effects of heat shock protein 70 on immature myocardium and myocardial interstitium in rabbits
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 19
- DOI:
- 10.3321/j.issn:1000-2790.2005.19.005
- Key Word:
- 热休克蛋白70;未成熟心肌;心肌间质;心肌保护
Abstract： 目的: 探讨热休克蛋白70(HSP70)对未成熟心肌和心肌间质的影响. 方法:健康新生长耳大白兔12只随机分为2组. 对照组(C组):ip生理盐水0.4 mL,24 h后取离体心脏,常规建立Langendorff离体心脏灌注模型,灌注15 min转为工作心15 min后停灌45 min,恢复灌注15 min改为工作心30 min;实验组(E组):ip去甲肾上腺素, 24 h后取离体心脏,方法同对照组. 测定心肌细胞中HSP70含量、血流动力学指标、心肌含水量(MWC)、心肌肌酸激酶(CK)和乳酸脱氢酶(LDH)漏出率、三磷酸腺苷(ATP)含量、超氧化物歧化酶(SOD)和丙二醛(MDA)含量、心肌组织羟脯氨酸(HP)含量、内皮素(ET) 含量、心肌细胞内Ca2+含量、心肌线粒体Ca2+-ATPase活性及其Ca2+含量、心肌线粒体合成ATP能力[ATP]m,心肌超微结构. 结果:E组HSP70含量明显高于C组(P<0.01);MWC低于C组(P<0.05);ATP含量、SOD活性、心肌线粒体Ca2+-ATPase活性、[ATP]m, HP含量优于C组(P<0.01),MDA含量、CK, LDH漏出率、心肌细胞内Ca2+含量、心肌线粒体Ca2+含量、ET含量低于C组(P<0.01),心肌超微结构损伤较C组明显减轻. 结论:HSP70对缺血再灌注未成熟心肌和心肌间质具有明显的保护作用.
- [1]A A, Knowlton.The role of heat shock proteins in the heart.[J].1995,27(1).
- [2]Fumikazu Nomura,Mitsuru Aoki,Joseph M Forbess,etc.Myocardial self-preservative effect of heat shock protein 70 on an immature lamb heart[J].1999,68(5).
- [3]R W, Currie,M, Karmazyn,M, Kloc,etc.Heat-shock response is associated with enhanced postischemic ventricular recovery.[J].1988,63.
- [4]X, Meng,J M, Brown,L, Ao,etc.Norepinephrine induces cardiac heat shock protein 70 and delayed cardioprotection in the rat through alpha 1 adrenoceptors.[J].1996,32.
- [5]RP Beckmann,LE Mizzen,WJ Welch.Interaction of Hsp 70 with newly synthesized proteins: implications for protein folding and assembly[J].1990,248 (4957).
- [6]Chong KY,Su CY,Lai CC,etc.Stable overexpression of the constitutive form of heat shock protein 70 confers oxidative protection.[J].1998,30(3).
- [7]M M, Hutter,R E, Sievers,V, Barbosa,etc.Heat-shock protein induction in rat hearts. A direct correlation between the amount of heat-shock protein induced and the degree of myocardial protection.[J].1994,89.
- [8]Corbett J,Latif N,Gray CC,etc.Relative induction of heat shock protein in coronary endothelial cells and cardiomyocytes: implications for myocardial protection.[J].1998,115(1).