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Construction and identification of sense and antisense human telomerase catalytic subunit adenovirus expression vector
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 19
- DOI:
- 10.3321/j.issn:1000-2790.2005.19.002
- Key Word:
- 人端粒酶催化亚单位;腺病毒;表达载体
Abstract： 目的: 构建hTERT正义和反义腺病毒表达载体. 方法: 用EcoRⅠ从pGRN145质粒上切下约3.5 kb的人端粒酶全长cDNA片段,然后连入pDC315质粒的EcoRⅠ酶切位点上,经BamHⅠ酶切鉴定出正义和反义表达载体,并对正反义重组质粒进一步测序鉴定其方向. 结果: 经BamHⅠ酶切后,正义质粒形成1.0+6.4 kb两条带. 反义重组质粒为1.0+2.5+3.9 kb三条带,与理论计算值完全一致,测序结果进一步确认了方向的正确性. 结论: 成功构建了hTERT的正、反义腺病毒表达载体.
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