You Position: Home > Paper
Generation of transgenic mouse founders of DSPP
( views:190, downloads:4 )
- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 16
- DOI:
- 10.3321/j.issn:1000-2790.2005.16.005
- Key Word:
- 牙;牙本质涎磷蛋白;小鼠转基因;微量注射
Abstract： 目的:构建四环素调控性牙本质涎磷蛋白(DSPP)转基因体系中的受控小鼠系.方法:从质粒pBluescript-DSPP中,将小鼠DSPP编码序列亚克隆到pTRE2的NotI/SalI位点间,构建转基因载体,经酶切鉴定和测序确认后,命名为pTRE2-DSPP;将XhoI酶切线性化的载体DNA纯化后,显微注射到小鼠受精卵的雄原核中,挑选生长状态好的受精卵,移植到假孕母鼠的输卵管.仔鼠出生3 wk后,用PCR及SouthernBlot检测阳性小鼠.结果:注射的受精卵共存活657枚,移卵至28只假孕母鼠后,产仔85只,PCR检测10只为阳性,Southern Blot检测6只为阳性.Southern Blot检测阳性小鼠分别传代,开始建系.结论:显微注射方法获得了DSPP转基因受控小鼠的GO代.
- [1]王捍国,肖明振,赵守亮,等.永生化人成牙本质细胞样细胞系的建立[J].2003,(10).
- [2]陆群,吴补领,周学东,等.小鼠牙髓干细胞培养及诱导分化[J].2003,(18).
- [3]孙汉堂.牙本质涎磷蛋白转基因小鼠模型的建立和相关研究[D].2003.
- [4]张蓉.牙本质涎磷蛋白在牙齿发育、矿化及牙髓损伤修复中作用的研究[D].2001.
- [5]Tsujigiwa H,Nagatsuka H,Cadena E,etc.The expression of dentin sialophosphoprotein gene in bone.[J].2002,81(6).
- [6]P, Papagerakis,A, Berdal,M, Mesbah,etc.Investigation of osteocalcin, osteonectin, and dentin sialophosphoprotein in developing human teeth.[J].2002,30(2).