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Effect of antisense oligodeoxynucleotide bcl-2 on expression of Bcl-2 protein and cell cycle of lens epithelial cells induced by galactose

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Author:
No author available
Journal Title:
JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
Issue:
16
DOI:
10.3321/j.issn:1000-2790.2005.16.004
Key Word:
基因;bcl-2;寡核苷酸类;反义;半乳糖;晶体/细胞学

Abstract: 目的:探讨反义bcl-2寡核苷酸(bcl-2 ASODN)对半乳糖诱导的兔晶状体中Bcl-2蛋白表达及细胞周期的影响.方法:人工合成与bcl-2基因翻译起始区序列互补的寡核苷酸并磷酸化修饰,用半乳糖和bcl-2 ASODN处理体外培养的兔晶状体,流式细胞术检测bcl-2 ASODN对半乳糖诱导的LEC中Bcl-2蛋白表达水平及细胞周期的影响.结果:5.0~15.0μmol/L反义bcl-2寡核苷酸能下调半乳糖诱导的晶状体上皮细胞中Bcl-2蛋白的表达,荧光强度从158.70±2.14降至113.94±2.88,同时可降低S期细胞数,从20.60%降至0.67%.结论:bcl-2 ASODN能抑制半乳糖诱导的晶状体中Bcl-2蛋白的表达水平,阻止细胞进入S期,抑制细胞增殖.

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