You Position: Home > Paper
Induction of secretion of interleukin-8 from lung epithelial cells by trypsin
( views:128, downloads:6 )
- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 16
- DOI:
- 10.3321/j.issn:1000-2790.2005.16.002
- Key Word:
- 胰蛋白酶;上皮细胞;白细胞介素-8;肺
Abstract： 目的:探讨胰蛋白酶对人上皮细胞白细胞介素-8(IL-8)分泌的影响.方法:人肺上皮细胞系A549细胞分别接种于12孔培养板各孔内,并分别用不同浓度的胰蛋白酶和/或胰蛋白酶抑制剂进行刺激.刺激时间为2 h、8 h和16 h.用ELISA方法检测上清液中的IL-8水平.结果:经过16 h的培养,胰蛋白酶可引起浓度相关性IL-8释放,胰蛋白酶在浓度1μg/L时就可引起IL-8的释放量增加,3 μg/L时诱导IL-8的释放量达高峰,为基础分泌量的5倍,但随着胰蛋白酶浓度的增加,IL-8的释放量反而下降.胰蛋白酶抑制剂可以抑制胰蛋白酶诱导IL-8释放的作用.时间相关曲线表明,胰蛋白酶从2 h起即可引起IL-8释放,16 h达高峰.结论:胰蛋白酶可促进人肺上皮细胞分泌IL-8,表明它能积极参与呼吸道的炎症过程.
- [1]Guo Sun,Martin A.Stacey,Luca Mori,etc.Interaction of Mite Allergens Der P3 and Der P9 with Protease-Activated Receptor-2 Expressed by Lung Epithelial Cells[J].2001,167(2).
- [2]T M, Cocks,B, Fong,J M, Chow,etc.A protective role for protease-activated receptors in the airways.[J].1999,398(6723).
- [3]Mapp CE,Braccioni F,Fabbri LM,etc.Expression of protease activated receptor-2 (PAR-2) in central airways of smokers and non-smokers.[J].2002,57(2).
- [4]Macfarlane SR,Seatter MJ,Kanke T,etc.Proteinase-activated receptors.[J].2001,53(2).
- [5]Philip J. Thompson,Anthony J. Bakker,Peter T. Graham,etc.Activation of Protease-Activated Receptor (PAR)-1, PAR-2, and PAR-4 Stimulates IL-6, IL-8, and Prostaglandin E_2 Release from Human Respiratory Epithelial Cells[J].2002,168(7).
- [6]Hollenberg MD,Moqbel R,Befus AD,etc.Airway epithelial cells release eosinophil survival-promoting factors (GM-CSF) after stimulation of proteinase-activated receptor 2.[J].2001,107(4).
- [7]Hollenberg MD,Vergnolle N,Wallace JL,etc.Protease-activated receptors in inflammation, neuronal signaling and pain.[J].2001,22(3).
- [8]Kahn ML,Hafezi-Moghadam A,Ley K,etc.Delayed onset of inflammation in protease-activated receptor-2-deficient mice.[J].2000,165(11).
- [9]Molino M.,Woolkalis M.,Numerof R.,etc.Interactions of Mast Cell Tryptase with Thrombin Receptors and Par-2[J].1997,272(7).
- [10]O'Brien PJ,Molino M,Kahn M,etc.Protease activated receptors: theme and variations.[J].2001,20(13).