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Effect of human β-globin MAR on transgene expression in different cells
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 14
- DOI:
- 10.3321/j.issn:1000-2790.2005.14.004
- Key Word:
- 核基质结合区;转基因;基因沉默;基因,报告
Abstract： 目的:研究人β-珠蛋白核基质结合区(matrix attachment region,MAR)在不同表达宿主中对转基因表达的影响.方法:通过PCR从人基因组扩增β-珠蛋白MAR,正向克隆至pCAT3-control载体SV40启动子的上游,分别检测在瞬时及稳定表达的情况下,MAR在NIH3T3及Hela细胞中对CAT报告基因的影响情况.结果:在瞬时表达情况下,NIH3T3细胞及Hela细胞中MAR均不能提高CAT基因的表达;在稳定整合的情况下,NIH3T3细胞中β-珠蛋白MAR可使CAT报告基因表达水平提高8倍,而在Hela细胞表达水平只提高2.5倍.结论:MAR能在一定程度上提高稳定外源基因的表达水平;MAR的作用与表达宿主相关.
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