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Effect of extracellular-regulated kinase signaling pathways on type Ⅰ collagen synthesis and TGF-β1 mRNA expression stimulated by acetaldehyde in rat hepatic stellate cells
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 13
- DOI:
- 10.3321/j.issn:1000-2790.2005.13.005
- Key Word:
- 肝纤维化;肝星状细胞;细胞外信号调节激酶;乙醛
Abstract： 目的:探讨细胞外信号调节激酶(Erk)信号传导通路调控乙醛刺激的肝星状细胞(HSCs)Ⅰ型胶原分泌及TGF-β1表达的影响.方法:用链霉蛋白酶和胶原酶原位灌流,Metrizamide密度梯度离心分离大鼠肝星状细胞,采用ELISA法定量测定PD98059阻断乙醛激活的肝星状细胞Erk活性后HSCs培养上清Ⅰ型胶原,RTPCR检测HSCs中TGF-β1mRNA.结果:PD98059在20μmol/L时可影响HSCsⅠ型胶原分泌及TGF-β1表达,但无统计学意义(P>0.05),50,100μmol/L抑制作用明显(P<0.05或P<0.01),呈剂量效应关系;TGF-β1表达与Ⅰ型胶原分泌呈正相关(r=0.93).结论:Erk信号传导通路是调控乙醛刺激的HSCsⅠ型胶原分泌及TGF-β1表达的重要通道,通过减少TGF-β1自分泌过程可能是PD98059抑制Ⅰ型胶原分泌的途径之一.
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