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Detection of transcription mediator CBF1 expression in mice bone tissue
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 12
- DOI:
- 10.3321/j.issn:1000-2790.2005.12.002
- Key Word:
- 基因表达调控;转录,遗传;CBF1;骨片成;基因表达
Abstract： 目的: 探讨转录调控因子CBF1(C-promoter binding factor-1, CBF1)在成鼠骨组织以及小鼠胚胎性骨组织的表达情况.方法: 采用实时RT-PCR方法检测包括骨组织在内的成年小鼠13种器官组织中CBF1的相对表达水平.采用组织原位杂交技术检测小鼠胚胎性趾骨、肋骨和椎骨中CBF1的分布.结果: 转录调控因子CBF1在成年小鼠各组织器官中广泛存在,在骨组织中检测到较高的CBF1表达水平.原位杂交分析显示: 发育中的趾骨和肋骨软骨细胞CBF1阳性染色,染色阳性程度与软骨细胞的分化程度有关;肋骨和椎骨骨化区周围成骨细胞呈强阳性染色;埋入骨基质的骨细胞则呈弱阳性染色.结论: 转录调控因子CBF1参与了小鼠骨组织的发育形成,可能也参与了成熟骨组织的改建和代谢.
- [1]J S, Mumm,R, Kopan.Notch signaling: from the outside in.[J].2000,228(2).
- [2]C, Oka,T, Nakano,A, Wakeham,etc.Disruption of the mouse RBP-J kappa gene results in early embryonic death.[J].1995,121(10).
- [3]Crowe R,Niswander L,Zikherman J.Delta-1 negatively regulates the transition from prehypertrophic to hypertrophic chondrocytes during cartilage formation.[J].1999,126(5).
- [4]S, Artavanis-Tsakonas,M D, Rand,R J, Lake.Notch signaling: cell fate control and signal integration in development.[J].1999,284(5415).
- [5]D G, Wilkinson,M A, Nieto.Detection of messenger RNA by in situ hybridization to tissue sections and whole mounts.[J].1993,225.
- [6]T Henkel,PD Ling,SD Hayward,etc.Mediation of Epstein-Barr virus EBNA2 transactivation by recombination signal-binding protein J kappa[J].1994,265 (5168).
- [7]F, Schweisguth,J W, Posakony.Suppressor of Hairless, the Drosophila homolog of the mouse recombination signal-binding protein gene, controls sensory organ cell fates.[J].1992,69(7).
- [8]D.J Dallas,P.G Genever,A.J Patton,etc.Localization of ADAM10 and notch receptors in bone[J].1999,25(1).
- [9]Taylor KL,Henderson AM,Hughes CC.Notch Activation during Endothelial Cell Network Formation in Vitro Targets the Basic HLH Transcription Factor HESR-1 and Downregulates VEGFR-2/KDR Expression.[J].2002,64(3).
- [10]Frederick M. Lu,Samuel E. Lux.Constitutively active human Notch1 binds to the transcription factor CBF1 and stimulates transcription through a promoter containing a CBF1-responsive element[J].1996,93(11).
- [11]Takahisa Furukawa,Shingo Maruyama,Masashi Kawaichi,etc.The Drosophila homolog of the immunoglobulin recombination signal-binding protein regulates peripheral nervous system development[J].1992,69(7).
- [12]Kabasawa Y,Perbal B,Miyawaki A,etc.The nephroblastoma overexpressed gene (NOV/ccn3) protein associates with Notch1 extracellular domain and inhibits myoblast differentiation via Notch signaling pathway.[J].2002,277(33).