You Position: Home > Paper
Regulation of PTA1 promoter by AP1
( views:233, downloads:20 )
- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 9
- DOI:
- 10.3321/j.issn:1000-2790.2005.09.003
- Key Word:
- PTA1;启动子;RACE;AP1
Abstract： 目的: 确定血小板/T细胞活化抗原1(PTA1)基因的转录起始点,及激活蛋白1(AP1)对PTA1启动子的调控作用. 方法: 采用5′-RACE方法确定PTA1基因的转录起始点;将含AP1的真核表达载体和含有PTA1启动子的荧光素酶的报告基因载体共转染人肝癌细胞系HHCC,培养48 h后检测其荧光素酶活性. 结果: 人PTA1基因的转录起始点定位于ATG上游229 bp处,AP1可以显著地增强PTA1启动子P1和P2的活性,转录因子ets1对AP1上调P1和P2活性有不同的调控作用. 结论:PTA1的转录起始点位于ATG上游229 bp处,AP1可能参与调控PTA1的表达.
- [1]菅金龙,朱勇,李德敏,等.人CD226基因SNP和启动子序列分析[J].2002,(3).
- [2]Michael Karin,Zheng-gang Liu,Ebrahim Zandi.AP-1 function and regulation[J].1997,9(2).
- [3]R S, Thomas,M J, Tymms,L H, McKinlay,etc.ETS1, NFkappaB and AP1 synergistically transactivate the human GM-CSF promoter.[J].1997,14(23).
- [4]S, Lepp,D, Bohmann.Diverse functions of JNK signaling and c-Jun in stress response and apoptosis.[J].1999,18(45).
- [5]J L, Scott,S M, Dunn,B, Jin,etc.Characterization of a novel membrane glycoprotein involved in platelet activation.[J].1989,264(23).
- [6]Shibuya K,Kojima H,Shibuya A,etc.CD226 mediates platelet and megakaryocytic cell adhesion to vascular endothelial cells.[J].2003,278(38).
- [7]W W, Lamph,P, Wamsley,P, Sassone-Corsi,etc.Induction of proto-oncogene JUN/AP-1 by serum and TPA.[J].1988,334(6183).
- [8]Marcenaro S,Cantoni C,Reymond N,etc.Identification of PVR (CD155) and Nectin-2 (CD112) as Cell Surface Ligands for the Human DNAM-1 (CD226) Activating Molecule.[J].2003,198(4).
- [9]Jin BQ.,Simmons D.,Aebersold RH.,etc.Tlisa1 (Pta1) Activation Antigen Implicated in T Cell Differentiation and Platelet Activation is a Member of the Immunoglobulin Superfamily Exhibiting Distinctive Regulation of Expression[J].1997,272(35).
- [10]G F, Burns,T, Triglia,J A, Werkmeister,etc.TLiSA1, a human T lineage-specific activation antigen involved in the differentiation of cytotoxic T lymphocytes and anomalous killer cells from their precursors.[J].1985,161(5).