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Construction and expression of siRNA against urokinase type plaminogen activator (uPA)
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- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 9
- DOI:
- 10.3321/j.issn:1000-2790.2005.09.002
- Key Word:
- siRNA;RNA干扰;尿激酶型纤溶酶原激活剂
Abstract： 目的:构建针对尿激酶型纤溶酶原激活剂(uPA)mRNA的siRNA的表达载体,并观察其对转染细胞中的uPA表达的抑制作用. 方法:化学合成用于产生针对uPA的发卡状RNA的寡核苷酸,各64个碱基,退火后插入线性化的pSUPER载体的H1启动子下游. 重组载体经限制性酶切和测序. 把构建的载体转染乳腺癌细胞系MDA-MB 231,观察其对uPA的干扰作用. 结果:限制性酶切和序列测定表明成功构建了可以表达针对uPA的发卡状RNA表达载体,该载体表达的发卡状RNA能够有效的下调uPA的表达. 结论:成功的构建了针对uPA的发卡状RNA表达载体,为进一步研究uPA的功能和肿瘤的基因治疗打下基础.
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