Effects of mifepristone administered at metaestrus on β-EP neurons in hypothalamus arcuate nucleus and anterior pituitary in rats
- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 5
- DOI:
- 10.3321/j.issn:1000-2790.2005.05.004
- Key Word:
- 米非司酮;β-内啡肽;弓状核;腺垂体
Abstract： 目的:探讨动情后期应用米非司酮对大鼠下丘脑弓状核和垂体β-内啡肽(β-EP)的影响. 方法:选择动情周期为4 d的大鼠40只,随机分为正常对照组(NC)和动情后期给药组(AM)各20只,采用免疫组化方法观察用药后的预期间情期和动情前期大鼠下丘脑弓状核和垂体β-EP的变化,以及卵巢和子宫内膜的形态学改变. 结果:①弓状核β-EP细胞计数显示:用药后在随后的预期间情期β-EP细胞数量高于对照组间情期数量(P<0.01),在随后的预期动情前期β-EP细胞数量亦高于对照组动情前期数量(P<0.01). ②垂体前叶β-EP细胞计数:用药后在随后的预期间情期β-EP细胞数量高于对照组间情期数量(P<0.05),在随后的预期动情前期β-EP细胞数量亦高于对照组动情前期数量(P<0.01). ③卵巢形态学变化:对照组间情期卵巢可见多个发育中的卵泡,直径较小,动情前期有数目较多的发育成熟的卵泡;动情后期给药组,在随后的预期间情期(24 h),发育中的卵泡少于对照组,直径较小,在预期的动情前期(48 h),发育卵泡仍然很少,直径较前变化不大,黄体较其他组大. ④对子宫内膜每横断面腺体数、腺腔直径、腺细胞高度和内膜厚度4参数的统计结果:动情后期给药组在随后的预期间情期(24 h),所有参数均高于对照组间情期(P<0.01);在随后的预期动情前期(48 h),所有数值均下降,子宫内膜厚度和腺细胞高度比对照组动情前期低(P<0.05);腺腔直径和腺体数与之无差异(P>0.05). 结论:动情后期单独应用米非司酮可影响大鼠下丘脑弓状核和垂体前叶β-EP神经元的数量,抑制相继的卵泡发育和延迟子宫内膜成熟. 动情后期应用米非司酮在一定剂量上影响性腺轴中枢部位,并可能进而影响卵巢和子宫内膜.
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