You Position: Home > Paper
Effect of bone marrow stem cells mobilization on heart function of acute myocardial ischemic rats
( views:296, downloads:4 )
- Author:
- No author available
- Journal Title:
- JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
- Issue:
- 4
- DOI:
- 10.3321/j.issn:1000-2790.2005.04.003
- Key Word:
- 骨髓祖代细胞;造血干细胞动员;急性病;心肌缺血
Abstract： 目的: 探讨联合应用粒细胞集落刺激因子(G-CSF)和干细胞因子(SCF)自体动员心肌梗死大鼠的骨髓干细胞对心功能的影响及其可能机制.方法: 雄性Wistar大鼠经结扎冠状动脉前降支制作心梗模型后分为2组.①动员组: 皮下注射 rhG-CSF 20 μg/kg, 2次/d,rh-SCF 200 μg/kg, 2次/d,共6 d; ②对照组: 皮下注射等量生理盐水,2次/d,共6 d.28 d后测定大鼠心率(HR)、左室缩-舒压差(LVEDP)、左室等容收缩/舒张期压力上升和下降最大速率(±LVdp/dtmax),通过伊文蓝-TTC染色方法观察心肌梗死范围的变化.结果: ①动员组左室缩-舒压差[(22.1±0.7) kPa]高于对照组[(20.9±0.9) kPa],左室压上升/下降最大速率[(590±15) kPa/s和(440±18) kPa/s]高于对照组[(567±18) kPa/s和(407±20) kPa/s],均具有统计学意义(P<0.05); ②动员组的缺血范围和心肌梗死的范围[(41±7)%和(43±6)%]比对照组[(53±8)%和(55±10)% ]均有所缩小,具有显著性差异(P<0.05).结论: G-CSF和SCF动员骨髓干细胞可以改善心肌梗死后大鼠的心脏功能.
- [1]高峰,施东伟,王晓明,等.极化液对缺血/再灌大鼠心肌细胞死亡及心脏功能的影响：胰岛素的关键作用[J].2003,(3).
- [2]陶凌,李源,高峰,等.缺血后处理对急性心肌缺血再灌注兔心脏的保护作用[J].2000,(6).
- [3]Schuster MD,Kocher AA,Burkhoff D,etc.Neovascularization of ischemic myocardium by human bone-marrow-derived angioblasts prevents cardiomyocyte apoptosis, reduces remodeling and improves cardiac function.[J].2001,7(4).
- [4]Piero, Anversa,Bernardo, Nadal-Ginard.Myocyte renewal and ventricular remodelling.[J].2002,415(6868).
- [5]Jackson KA,Mi T,Goodell MA.Hematopoietic potential of stem cells isolated from murine skeletal muscle (see comments)[J].1999,96(25).
- [6]S, Tomita,R K, Li,R D, Weisel,etc.Autologous transplantation of bone marrow cells improves damaged heart function.[J].1999,100.