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Generation of H/R-S-like murine cells by LMP1 overexpression in A20

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Author:
No author available
Journal Title:
JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY
Issue:
4
DOI:
10.3321/j.issn:1000-2790.2005.04.002
Key Word:
霍奇金病;里德-斯特恩伯格细胞潜伏膜蛋白1;A20

Abstract: 目的: 研究EB病毒潜伏膜蛋白1(LMP1)转染小鼠B淋巴瘤细胞A20能否诱导出霍奇金淋巴瘤H/R-S样细胞.方法: 采用RT-PCR方法获得LMP1全外显子片段 ,使之克隆到真核表达载体PLXSN中,限制性酶切、PCR扩增和测序鉴定重组载体;运用脂质体介导转染技术,将LMP1的真核表达重组质粒和空载体质粒导入A20细胞.结果: 扩增的LMP1cDNA长度为 1.1 kb,测序结果与已知序列吻合,重组真核表达质粒经酶切鉴定表明获得正确重组子;经G418筛选获得稳定整合了LMP1和空载体的亚克隆细胞,Western Blot印迹鉴定PLXSN-LMP1组有LMP1蛋白的表达,出现了H/R-S样细胞形态改变.结论: 建立了小鼠A20RS样细胞模型,为下一步建立霍奇金淋巴瘤动物模型,研究其发病机制奠定了基础.

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